This work studied the mechanical properties and antibacterial efficacies of natural rubber (NR) reinforced with different grades of carbon black N220, N330 and N660 at various loadings 0, 20, 40 and 60 parts per hundred of rubber (phr). 2-Hydroxypropyl-3-Piperazinyl-Quinoline carboxylic acid Methacrylate (HPQM) based Neusilin at loading 0, 3, 5 and 7 phr were used as antibacterial agent against Escherichia coli (E. coli) ATCC 25923 and Staphylococcus aureus (S. aureus) ATCC 25922. The Inhibition zone (Halo test) and Plate Count Agar (PCA) methods were employed to assess the anti-bacterial ability. The experimental results suggested that carbon black grade N330 loadings at 40 phr in NR vulcanized improved the tensile modulus, hardness and tensile strength. However, the overall mechanical properties of the NR vulcanizates were not affected by the addition of HPQM based Neusilin. HPQM based Neusilin addition in NR vulcanized reinforced with carbon black against E. coli was found to be more effective than that against S. aureus. The antibacterial efficacy was conversely associated with the reinforcement level of the NR vulcanizates by carbon black, the higher the reinforcement level the poorer the antibacterial ability.