Polyhydroxyalkanoates Production from Fatty Acids of Palm Oil using Pseudomonas fluorescens TISTR 358
Tatiya Bhandasak 1 Pusacha Ponchumni 1 Pinanong Tanikkul 2 Vanatpornratt Sawasdee 3 and Nipon Pisutpaisal 1,2*
1 Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut University of Technology North Bangkok.
2 The Biosensor and Bioelectronics Technology Centre, King Mongkut University of Technology North Bangkok.
3 Department of Environmental Studies, College of Innovative Management, Valaya Alongkorn Rajabhat University under The Royal Patronage.


This study aimed to investigate the production of polyhydroxyalkanoates (PHAs) from fatty acids of saponified palm oil as a carbon source using Pseudomonas fluorescens TISTR 358. The batch experiments were setup in an incubator shaker with 180 rpm at 30ºC. Varying the concentrations of fatty acids including 0.50, 0.75, 1.00 and 1.50% (w/v), respectively and initial pH 7.0. The results showed that the concentrations of carbon sources influenced cell growth, pH and the PHAs production. The maximum of cell dry weight were 1.63, 1.60, 1.08 and 1.05 g L-1 which were observed in fatty acids concentrations of 0.50, 0.75, 1.00 and 1.50% (w/v), respectively at 48 hrs incubation. The highest PHAs content after 72 hrs incubation was 12.61% (0.17 g L-1) of fatty acids concentrations was 0.50% (w/v). The microbial cells showed high red fluorescent, when the cells were determined using the fluorescent dye Nile red and the PHAs granule of intracellular the microbial cell were seen by transmission electron microscope. The results indicated that P. fluorescens TISTR 358 could produce PHAs in an intracellular by using fatty acids from palm oil.

Keywords : Polyhydroxyalkanoates, Pseudomonas fluorescens, Palm oil, Fatty acids